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rat anti tubulin alpha antibody mca 78g  (Bio-Rad)


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    Structured Review

    Bio-Rad rat anti tubulin alpha antibody mca 78g
    Strains with or without Myc-tagged orf19.3456 and HA-tagged orf19.193 were grown to log phase in YPD at 30°C. Protein extracts were prepared as described in materials and methods and immunoprecipitated with an anti-Myc antibody. The immunoprecipitated orf19.3456 -encoded kinase was detected by Western blotting with an anti-Myc antibody, and the co-immunoprecipitated orf19.193 -encoded protein with an anti-HA antibody (top panels, IP). Tagged proteins in the input samples were detected with anti-HA and anti-Myc antibodies (bottom panels, input). Detection of tubulin with <t>an</t> <t>anti-tubulin</t> antibody served as loading control for the input samples. Both independently generated series of strains were used for the experiment.
    Rat Anti Tubulin Alpha Antibody Mca 78g, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 941 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+tubulin+alpha+antibody+mca+78g/pmc13128113-288-19-25?v=Bio-Rad
    Average 96 stars, based on 941 article reviews
    rat anti tubulin alpha antibody mca 78g - by Bioz Stars, 2026-07
    96/100 stars

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    1) Product Images from "Inducible gene deletion reveals essentiality of protein kinases and a septation initiation network in Candida albicans"

    Article Title: Inducible gene deletion reveals essentiality of protein kinases and a septation initiation network in Candida albicans

    Journal: PLOS Genetics

    doi: 10.1371/journal.pgen.1012118

    Strains with or without Myc-tagged orf19.3456 and HA-tagged orf19.193 were grown to log phase in YPD at 30°C. Protein extracts were prepared as described in materials and methods and immunoprecipitated with an anti-Myc antibody. The immunoprecipitated orf19.3456 -encoded kinase was detected by Western blotting with an anti-Myc antibody, and the co-immunoprecipitated orf19.193 -encoded protein with an anti-HA antibody (top panels, IP). Tagged proteins in the input samples were detected with anti-HA and anti-Myc antibodies (bottom panels, input). Detection of tubulin with an anti-tubulin antibody served as loading control for the input samples. Both independently generated series of strains were used for the experiment.
    Figure Legend Snippet: Strains with or without Myc-tagged orf19.3456 and HA-tagged orf19.193 were grown to log phase in YPD at 30°C. Protein extracts were prepared as described in materials and methods and immunoprecipitated with an anti-Myc antibody. The immunoprecipitated orf19.3456 -encoded kinase was detected by Western blotting with an anti-Myc antibody, and the co-immunoprecipitated orf19.193 -encoded protein with an anti-HA antibody (top panels, IP). Tagged proteins in the input samples were detected with anti-HA and anti-Myc antibodies (bottom panels, input). Detection of tubulin with an anti-tubulin antibody served as loading control for the input samples. Both independently generated series of strains were used for the experiment.

    Techniques Used: Immunoprecipitation, Western Blot, Control, Generated



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    96
    Bio-Rad rat anti tubulin alpha antibody mca 78g
    Strains with or without Myc-tagged orf19.3456 and HA-tagged orf19.193 were grown to log phase in YPD at 30°C. Protein extracts were prepared as described in materials and methods and immunoprecipitated with an anti-Myc antibody. The immunoprecipitated orf19.3456 -encoded kinase was detected by Western blotting with an anti-Myc antibody, and the co-immunoprecipitated orf19.193 -encoded protein with an anti-HA antibody (top panels, IP). Tagged proteins in the input samples were detected with anti-HA and anti-Myc antibodies (bottom panels, input). Detection of tubulin with <t>an</t> <t>anti-tubulin</t> antibody served as loading control for the input samples. Both independently generated series of strains were used for the experiment.
    Rat Anti Tubulin Alpha Antibody Mca 78g, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+tubulin+alpha+antibody+mca+78g/pmc13128113-288-19-25?v=Bio-Rad
    Average 96 stars, based on 1 article reviews
    rat anti tubulin alpha antibody mca 78g - by Bioz Stars, 2026-07
    96/100 stars
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    Strains with or without Myc-tagged orf19.3456 and HA-tagged orf19.193 were grown to log phase in YPD at 30°C. Protein extracts were prepared as described in materials and methods and immunoprecipitated with an anti-Myc antibody. The immunoprecipitated orf19.3456 -encoded kinase was detected by Western blotting with an anti-Myc antibody, and the co-immunoprecipitated orf19.193 -encoded protein with an anti-HA antibody (top panels, IP). Tagged proteins in the input samples were detected with anti-HA and anti-Myc antibodies (bottom panels, input). Detection of tubulin with an anti-tubulin antibody served as loading control for the input samples. Both independently generated series of strains were used for the experiment.

    Journal: PLOS Genetics

    Article Title: Inducible gene deletion reveals essentiality of protein kinases and a septation initiation network in Candida albicans

    doi: 10.1371/journal.pgen.1012118

    Figure Lengend Snippet: Strains with or without Myc-tagged orf19.3456 and HA-tagged orf19.193 were grown to log phase in YPD at 30°C. Protein extracts were prepared as described in materials and methods and immunoprecipitated with an anti-Myc antibody. The immunoprecipitated orf19.3456 -encoded kinase was detected by Western blotting with an anti-Myc antibody, and the co-immunoprecipitated orf19.193 -encoded protein with an anti-HA antibody (top panels, IP). Tagged proteins in the input samples were detected with anti-HA and anti-Myc antibodies (bottom panels, input). Detection of tubulin with an anti-tubulin antibody served as loading control for the input samples. Both independently generated series of strains were used for the experiment.

    Article Snippet: For the detection of tubulin, membranes were blocked with 5% milk in TBST and incubated overnight at 4°C with rat anti-tubulin alpha antibody MCA 78G (Bio-Rad), washed with TBST, and then incubated with rabbit anti-rat HRP-conjugated antibody STAR21B (Bio-Rad).

    Techniques: Immunoprecipitation, Western Blot, Control, Generated